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Image Search Results
Journal: British Journal of Cancer
Article Title: Emergence of resistance to tyrosine kinase inhibitors in non-small-cell lung cancer can be delayed by an upfront combination with the HSP90 inhibitor onalespib
doi: 10.1038/bjc.2016.294
Figure Lengend Snippet: Characterisation of ex vivo crizotinib-resistant cell lines. ( A ) Circos plot displaying nonsynonymous somatic mutations and copy number variations in H2228-CR2, -CR4 and -CR6 samples (compared with H2228-CS) that were analysed after exome sequencing. Mutations are displayed as coloured radial lines and those predicted as functional are labelled. The inner ring displays copy number data for each sample. Zoomed chromosome 2 is displayed in the top left section. ( B ) Analysis of ALK signalling pathway by western blot in ex vivo H2228 cell lines (CR=crizotinib resistant; CS=crizotinib sensitive). ( C ) Measure of the basal level of pALK in H2228-CS and -CR cell lines by ELISA.
Article Snippet: The DNA isolation and
Techniques: Ex Vivo, Sequencing, Functional Assay, Western Blot, Enzyme-linked Immunosorbent Assay
Journal: Urology
Article Title: CDC73 germline mutation in a family with mixed epithelial and stromal tumors
doi: 10.1016/j.urology.2018.11.013
Figure Lengend Snippet: (A) Copy number (Depth ratio) and allelic imbalance (B allele frequency) for chromosome 1 (top) and the entire genome in the largest tumor from patient III:2 were evaluated by whole exome sequencing. While no copy loss for chromosome 1 was observed, the allelic imbalance (B allele frequency) analysis demonstrated a significant loss of allelic variation in most of the qter arm of chromosome 1, including the CDC73 locus, indicating a copy neutral LOH event. (B) Copy number analysis was confirmed using quantitative PCR of selected exons (1, 7, 10, 11, 14 and 17) spread throughout the length of the CDC73 gene. The gene structure for CDC73 and location of the primers is indicated. Copy number was normalized to blood DNA from patient III:2 and a normal copy number pattern was observed in both tumors from patient III:2.
Article Snippet: ClinOmics Analysis: DNAs from a tumor and matched normal kidney tissue from Patient III:2 were subjected to whole
Techniques: Sequencing, Real-time Polymerase Chain Reaction